Meredith Lab

Protocols

TRANSGENIC MOUSE LINES

     Transnetyx (automated assays for genotyping our transgenic lines)

     Kcnma1–/– mice (global BK channel knockout)

Description of Kcnma1-/- mice:  Meredith AL, Thorneloe KS, Werner ME, Nelson MT, and Aldrich RW (2004). Overactive bladder and incontinence in the absence of the BK Ca2+-activated K+ channel.  Journal of Biological Chemistry 279:36746-36752.
http://www.ncbi.nlm.nih.gov/pubmed/15184377

          PCR Genotyping Protocol for Kcnma1–/–

Tg-BKR207Q  mice (gain-of-function BK channel transgenic)

Kcnma1fl (conditional BK channel knockout, coming soon)

SUPPLEMENTAL DATA

     Mis-expression of the BK K+ channel disrupts suprachiasmatic nucleus circuit rhythmicity and alters clock-controlled behavior.  AJP- Cell Physiology 304: C299-C311 (2013).

GENBANK CLONES (Genbank Accession Links Coming Soon)

Tg-BKR207Q  (JX429072)

– SCN Variant SCN-C5b (JX462785)

– SCN Variant SCN-C5b:YFP (JX462786)

– mbr5